University of Delaware, 163 The green, 177 Brown Laboratory, Newark, DE, 19716
Wednesday, November 1, 2017 - 15:45 to 16:45
The majority of drug targets are membrane proteins, because they control and mediate fundamental life processes. Bacterial chemotaxis proteins sense and control responses to the environment, and are potential targets for novel antibiotics. Chemotaxis receptors operate in the cell in a complex with the CheA kinase and the CheW coupling protein, which together form remarkable signaling arrays that extend 200 nm in the membrane. We are combining biochemical methods for the assembly of functional, native-like arrays with solid-state NMR and hydrogen exchange mass spectrometry to investigate how the receptor structure and dynamics change during signaling. Our results provide insights into the mechanism of transmembrane signaling and approaches for investigating mechanisms of other proteins within the large multi-protein complexes that are formed in cells.