Lentiviruses employ cellular metabolites to regulate infection

The structural polyprotein Gag is conserved among all retroviruses and mediates virus assembly via oligomerization into incomplete lattices that are stabilized by dimeric, trimeric and hexameric contacts. Despite a high degree of conservation at the secondary and tertiary structure level, the quaternary interactions between the CA domains of retroviral Gag vary. Recently, the small cellular molecule IP6 was identified as an assembly co-factor of the lentivirus HIV-1. To better understand the structural variability of retroviruses and to determine if IP6 is an assembly cofactor of other lentiviruses we determined the structure of the HIV-1 related retrovirus EIAV. Using cryo-electron tomography and subtomogram averaging, in vitro assembly, mutation analysis, and molecular dynamic simulations, we determined and characterized the structure of the EIAV immature lattice. Furthermore, we found that IP6 is an assembly cofactor of EIAV, and other lentiviruses.

Read the manuscript: PLoS Pathogens

The key to unlock Chlamydia fusion

Taken from: https://phys.org/news/2019-06-key-bacterial-fusion.html

Most people know Chlamydia as the venereal disease that can cause infertility if left untreated. But for researchers studying the causative agent, Chlamydia trachomatis, it's a bacteria with intriguing properties. Rather than grow and replicate in the blood or other bodily fluids, C. trachomatis get inside cells where they multiply. In most people, this trait keeps the bacterium from being detected by the immune system, and helps the disease fly under the radar; not everyone infected with Chlamydia will show symptoms of the disease. But managing to stay alive inside an infected cell is no small feat for bacteria.

A synergistic collaboration between computational and experimental scientist have shed light in the mechanism employe dby C. trachomatis to fuse in the interior of living cells. The ability of C. trachomatis to fuse is directly related to its pathogenicity and therefore a deeper knowledge of the molecular mechanisms enables new target for the development of new therapeutic leads.

Deanna Greco is awarded the 2019 Barry Goldwater Scholarship for work in our lab

Undegraduate student Deanna Greco (class of 2020') has been awarded the Barry Goldwater Scholarship (https://goldwater.scholarsapply.org/2019-goldwater-scholars-by-instituti...). 

Alex Bryer wins poster competition at BPS-19

Second year graduate student Alex Bryer has won the poster competition at the Biophysical Society Meeting.

Our group reveals details of how HIV-1 becomes infectious.

The article “Dynamic Regulation of HIV-1 Capsid Interaction with the Restriction Factor TRIM5α” was published Oct. 17 in the Proceedings of the National Academy of Sciences.

In addition to Quinn, Polenova and Perilla, the authors are Mingzhang Wang, Matthew P. Fritz, Brent Runge and Chaoyi Xu, all doctoral students at UD, and Jinwoo Ahn and Angela M. Gronenborn of the Pittsburgh Center for HIV Protein Interactions and the Department of Structural Biology at the University of Pittsburgh School of Medicine.

Gronenborn is a University of Pittsburgh School of Medicine Rosalind Franklin Professor and chair of the Department of Structural Biology. She is the director of the National Institutes of Health-funded Pittsburgh Center for HIV Protein Interactions, which brings together high-caliber scientists and facilities to study the HIV virus and its interactions with host cell proteins.

Our group is awarded the best HPC collaboration at SuperComputing 18

Our group has been recognized in the annual HPCwire
Readers’ and Editors’ Choice Awards, presented at the 2018 International Conference for High Performance
Computing, Networking, Storage and Analysis (SC18), in Dallas, Texas. Specifically we have been awarded the price for our collaborations with researchers at the Pittsburgh Center for HIV Protein Interactions, and in collaboration with D.E. Shaw Research and PSC, discover how drugs stop HIV maturation (Nature Communications).

The coveted annual HPCwire Readers’ and Editors’ Choice Awards are determined through a

nomination and voting process with the global HPCwire community, as well as selections from the HPCwire
editors. The awards are an annual feature of the publication and constitute prestigious recognition from the HPC
community. These awards are revealed each year to kick off the annual supercomputing conference, which
showcases high performance computing, networking, storage, and data analysis.

Our work is nominated to the HPC Wire reader's award

These annual awards are a way for our community to recognize the best and brightest innovators within the global HPC community.

We were nominated in the Best HPC Collaboration (Academia/Government/Industry) by the Pittsburgh Super Computing center.

Work performed by Juan R. Perilla, has previously won the 2013 HPCWire Reader's award for Best Use of HPC Application in Life Sciences.

The 2018 Categories Include the Following:

* Best Use of HPC Application in Life Sciences
* Best Use of HPC in Physical Sciences
* Best Use of HPC in Manufacturing
* Best Use of HPC in Energy
* Best Use of HPC in Automotive
* Best Use of HPC in Entertainment
* Best Use of HPC in Financial Services
* Best Use of High Performance Data Analytics
* Best Use of AI (Deep Learning/Machine Learning)
* Best Use of HPC in the Cloud (Use Case)
* Best Cloud Platform for HPC
* Best HPC Server Product or Technology
* Best HPC Visualization Product or Technology
* Best HPC Interconnect Product or Technology
* Best HPC Programming Tool or Technology
* Best HPC Cluster Solution or Technology
* Best AI Product or Technology
* Top 5 New Products or Technologies to Watch
* Best HPC Collaboration (Academia/Government/Industry)
* Top Supercomputing Achievement
* Top HPC-Enabled Scientific Achievement
* Top Energy-Efficient HPC Achievement
* Top 5 Vendors to Watch
* Workforce Diversity Leadership Award
* Outstanding Leadership in HPC (Individual)

Nominations are accepted from readers, users, vendors – virtually anyone who is connected to the HPC community and is a reader of HPCwire.

Supercomputer Simulations show new target in HIV-1 replication

Scientists from the group found the naturally-occurring compound a hexakisphosphate (IP6) promotes both assembly and maturation of HIV-1. We discovered, in collaboration with other researchers, that HIV uses this small molecule to complete its function. This is a molecule that's extremely available in human cells and in other mammalian cells. HIV has evolved to make use of these small molecules present in our cells to essentially be infectious. Our study was published in the journal Nature in August 2018.

More information.

Postdoctoral researcher position

The Department of Chemistry & Biochemistry at the University of Delaware invites applications for a Postdoctoral Researcher position beginning September 1, 2018 (a later start can be negotiated). The primary function of this position is to conduct research regarding the molecular architecture of the HIV-1 virus in the research group of Prof. Juan R. Perilla (http://perilla.chem.udel.edu).  The U.S. National Institutes of Health-funded project is on the design, development and execution of computer simulations of biomedically relevant systems including, but not limited to, the HIV-1 virus. Preference will be given to applicants with expertise in 1) protein dynamics; 2) molecular dynamics simulations; and 3) high-performance computing environments. Prior programming experience is preferred but not required. The Postdoctoral Researcher will design, perform, and develop new scientific tools for the analysis of long-time and/or large-scale molecular dynamics simulations in collaboration with the PI. The selected candidate will also participate in the development, testing and implementation of novel computational methods, the preparation of technical reports and peer-reviewed publications, and the dissemination of project results at professional meetings.  Within the Department of Chemistry and Biochemistry, the successful candidate will benefit from excellent core laboratories and a vibrant and supportive intellectual environment.

Applicant must hold a PhD in biophysics, biochemistry, chemistry, physics, or related area.  Potential applicants are strongly encouraged to contact Prof. Juan R. Perilla (biophysics-positions@udel.edu) prior to submission.  Candidates should submit a cover letter, CV, and the names, phone numbers and email addresses of three references through the University of Delaware Jobs website.  The initial appointment is for one year with the possibility of renewal, based on the availability of funding and contingent upon satisfactory performance.  This position includes full UD benefits (www.udel.edu/benefits).  Review of applications will begin immediately and will continue until the position is filled.

Apply here

The biophysical society spoke with us on the world hepatitis day

July 28 is World Hepatitis Day. The Biophysical Society spoke with us regarding our work on Hepatitis B. Hepatitis B is a potentially life-threatening liver infection caused by the hepatitis B virus (HBV). It can cause chronic infection and puts people at high risk of death from cirrhosis and liver cancer. According to the World Health Organization, an estimated 257 million people are living with hepatitis B virus infection. 

More on the society's blog :  Biophysics on world hepatitis day.